ABSTRACT
Background: Pseudomonas aeruginosa [P. aeruginosa] is one of the most common causative bacteria in chronic wound infection. It is increasingly becoming resistant to many anti-pseudomonal agents. So, it is necessary to advance the field of alternative treatment. Probiotics are viable, non-pathogenic microorganisms that alter the micro-flora of the gastro intestinal tract of the host, yielding a positive influence on health and body physiology
Objective: To investigate the in-vitro effect exerted by Lactobacillus plantarum [L. plantarum] on the antibiotic sensitivity of P. aeruginosa
Methodology: This study was carried out in Medical Microbiology and Immunology Department, Faculty of Medicine, Tanta University on forty patients admitted, during the period of research [February 2017 to August 2017]. P. aeruginosa isolates were identified by results of culture and conventional biochemical reactions. The effect of L.plantarum on antibiotic sensitivity of P. aeruginosa was detected by measuring the antibiotic sensitivity of the isolated strains of P. aeruginosa before and after addition of L.plantarum
Results: Among the forty wound samples investigated, the most predominant bacterial isolate was P. aeruginosa [50%]. P. aeruginosa infection was more common in males than females with predominance in old age. P.aeruginosa susceptibility pattern to different antimicrobial agents significantly increased after addition of L. plantarum. 100% of isolates became sensitive to colistin sulphate followed by imipenem [70%], ciprofloxacin [65%], pipracillintazobactam [60%], gentamicin [35%], aztreonam [30%] and pipracillin [20%]
Conclusion: P. aeruginosa was the predominant bacterial isolate in the studied wound infections. P. aeruginosa susceptibility pattern to different antimicrobial agents significantly increased after addition of L. plantarum
ABSTRACT
Despite the growing understanding of the involvement of protooncogenes and tumour suppressor genes in the oncogenesis of CRC, the exact biological and molecular mechanisms underpinning this process remain poorly understood. The signal transducer and activator of transcription [STAT3] has been implicated in the regulation of growth and malignant transformation. Accumulating evidences have come to indicate that abnormalities in the Janus kinase [JAK]/STAT pathway are involved in oncogenesis of several cancers. The aim of this study was to investigate the expression of JAK3 and STAT3 in both normal and activated forms by immunohistochemistry in adenomas of the colon, ulcerative colitis and CRC compared to normal colonic mucosa. Tissues from 30 cases with primary CRC and seven cases with ulcerative colitis [UC], removed by colectomy, were included. In addition, tissues from 10 colonic adenomas, 15 CRC and eight cases with UC, obtained by endoscopic biopsies, were examined histopathologically. Immuno-histochemical evaluation of STAT3, p-STAT3, JAK3 and p-JAK3 expression in tissue sections was completed. Statistical analysis and correlation of data were then performed. Normal colonic mucosa showed expression of STAT3 only. Immunoreactivity of p-JAK3 increased significantly [p < 0.05] and correlated with the degree of dysplasia in colonic adenomas. Immunoreactivity of p-STAT3 increased significantly [p < 0.05] and correlated with the degree of dysplasia in cases with UC. In CRC a significant positive correlation was found between p-STAT3 expression and grading, STAT3, JAK3 and p-JAI<3 and TNM or Dukes' staging, and p-STAT3 and nodal status excluding distant metastasis [p<0.05]. JAK3 and STAT3, and particularly their activated forms, were found to correlate significantly with the degree of dysplasia in adenomas and UC, indicating their potential role in colorectal carcinogenesis. They also correlate with anaplasia and invasion, suggesting a definitive role in progression of CRC